Affiliation:
1. Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Abstract
ABSTRACT
We have isolated new histone H3 mutants in
Saccharomyces cerevisiae
that confer phenotypes indicative of transcriptional defects. Here we describe the characterization of one such mutant, encoded by the
hht2-11
allele, which contains the single amino acid change L61W in the globular domain of H3. Whole-genome expression analyses show that the
hht2-11
mutation confers pleiotropic transcriptional defects and that many of the genes it affects are normally controlled by the Swi/Snf chromatin remodeling complex. Furthermore, we show that Swi/Snf occupancy at two promoters,
PHO84
and
SER3
, is reduced in
hht2-11
mutants. Detailed studies of the
PHO84
promoter suggest that the
hht2-11
mutation impairs Swi/Snf association with chromatin in a direct fashion. Taken together, our results strongly suggest that the integrity of the globular domain of histone H3 is an important determinant in the ability of Swi/Snf to associate with chromatin.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
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