Murine Monoclonal Antibodies Specific for Lipopolysaccharide of Escherichia coli O26 and O111

Author:

Rivera-Betancourt Mildred1,Keen James E.1

Affiliation:

1. Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, United States Department of Agriculture, Clay Center, Nebraska 68933

Abstract

ABSTRACT Monoclonal antibody (MAb) 12F5 reacted with 35 Escherichia coli O26 isolates and cross-reacted with 1 of 365 non- E. coli O26 isolates. MAb 15C4 reacted with 30 E. coli O111 strains and 8 Salmonella O35 strains (possessing identical O antigen) but not with 362 other bacterial strains. Lipopolysaccharide immunoblots confirmed MAb O-antigen specificity.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference34 articles.

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3. Shiga-like toxin-producing Escherichia coli O111 and associated hemolytic-uremic syndrome: a family outbreak;Banatvala N.;Pediatr. Infect. Dis. J.,1996

4. Molecular cloning and expression in Escherichia coli K-12 of the rfb gene cluster determining the O antigen of an E. coli O111 strain;Bastin D. A.;Mol. Microbiol.,1991

5. Immunoprotective murine monoclonal antibodies specific for the outer-core polysaccharide and for the O-antigen of Escherichia coli O111:B4 lipopolysaccharide (LPS);Coughlin R. T.;J. Immunol.,1987

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