Nucleotide Sequence Requirements at the 5′ End of the Influenza A Virus M RNA Segment for Efficient Virus Replication-Reference-Cited by-同舟云学术

Nucleotide Sequence Requirements at the 5′ End of the Influenza A Virus M RNA Segment for Efficient Virus Replication

Published:2009-04 Issue:7 Volume:83 Page:3384-3388
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Ozawa Makoto¹²,Maeda Junko³,Iwatsuki-Horimoto Kiyoko¹,Watanabe Shinji²,Goto Hideo¹,Horimoto Taisuke¹,Kawaoka Yoshihiro¹⁴⁵²

Affiliation:

1. Division of Virology, Department of Microbiology and Immunology

2. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706

3. Laboratory of Prion Diseases, Department of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-818, Japan

4. International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan

5. ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama 332-0012, Japan

Abstract

ABSTRACT The mechanism by which the influenza A virus genome is packaged into virions is not fully understood. The coding and noncoding regions necessary for packaging of the viral RNA segments, except for the M segment, have been identified. Here, we delineate the M segment regions by incorporating a reporter viral RNA into virions and by generating viruses possessing mutations in the regions. We found that, like the other segments, the M segment coding regions are essential for virion incorporation and that the nucleotide length rather than the nucleotide sequence of the 5′ end of the coding region is important.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.02513-08

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