Hemoglobin and Escherichia coli , a Lethal Intraperitoneal Combination

Author:

Bornside George H.1,Bouis Pierre J.1,Cohn Isidore1

Affiliation:

1. Department of Surgery, School of Medicine, Louisiana State University, New Orleans, Louisiana 70112

Abstract

Intraperitoneal injection into mice of approximately 8 × 10 6 washed cells of Escherichia coli suspended in a lysate of washed human red blood cells or an aqueous solution of crystalline hemoglobin was lethal. E. coli suspended in washed intact erythrocytes, whole blood, plasma, or saline was innocuous. Fractionation of non-hemoglobin proteins from hemoglobin in lysates showed that only hemoglobin promoted a lethal infection. Overwhelming intraperitoneal growth of E. coli was attained in about 12 hr in lethal infections. The polymorphonuclear leukocytic response was ineffective against this rapid growth. The lethal mechanism is hypothesized to center on a unique role for free hemoglobin in inhibiting peritoneal absorption and stimulating an intraperitoneal exudate which supports luxuriant bacterial growth. Death is attributed to a lethal intoxication from bacterial endotoxins. This role for hemoglobin involves neither enhanced bacterial virulence nor lowered host resistance, and it would be of importance not only in peritonitis but also in problems where hemolysis and infection coexist.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference23 articles.

1. Staphylococcal enhancement of susceptibility to bacterial infection in the mouse;ARNDT W. F.;J. Infect. Diseases,1963

2. Contribution of serum and cellular factors in host defense reactions;TEN K. F.;New Engl. J. Med.,1963

3. Hemoglobin as a bacterial virulence-enhancing factor in fluids produced in strangulation intestinal obstruction;BORNSIE G. H.;Am. Surgeon,1968

4. LETHALITY OF HEMOGLOBIN AND E. COLI 4. BORNSIDE, G. H;VOL;Proc. Soc. Exptl. Biol. Med.,1968

5. Abolition of passive immunity to bacterial infections by iron;BULLEN J. J.;Nature,1967

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3