Complete Structure of the Enterococcal Polysaccharide Antigen (EPA) of Vancomycin-Resistant Enterococcus faecalis V583 Reveals that EPA Decorations Are Teichoic Acids Covalently Linked to a Rhamnopolysaccharide Backbone

Author:

Guerardel Yann1ORCID,Sadovskaya Irina2ORCID,Maes Emmanuel1ORCID,Furlan Sylviane3,Chapot-Chartier Marie-Pierre3ORCID,Mesnage Stéphane4ORCID,Rigottier-Gois Lionel3ORCID,Serror Pascale3ORCID

Affiliation:

1. Univ. Lille, CNRS, UMR 8576—UGSF—Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France

2. Univ. Littoral Côte d’Opale, UMR 1158 BioEcoAgro, TERRA Viollette, USC Anses, INRAe, Univ. Lille, Univ. Artois, Univ. Picardie Jules Verne, Univ. Liège, Yncréa, Boulogne-sur-Mer, France

3. Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France

4. University of Sheffield, Department of Molecular Biology and Biotechnology, Sheffield, United Kingdom

Abstract

Enterococci are opportunistic pathogens responsible for hospital- and community-acquired infections. All enterococci produce a surface polysaccharide called EPA ( e nterococcal p olysaccharide a ntigen) required for biofilm formation, antibiotic resistance, and pathogenesis. Despite the critical role of EPA in cell growth and division and as a major virulence factor, no information is available on its structure. Here, we report the complete structure of the EPA polymer produced by the model strain E. faecalis V583. We describe the structure of the EPA backbone, made of a rhamnan hexasaccharide substituted by Glc and GlcNAc residues, and show that teichoic acids are covalently bound to this rhamnan chain, forming the so-called “EPA decorations” essential for host colonization and pathogenesis. This report represents a key step in efforts to identify the structural properties of EPA that are essential for its biological activity and to identify novel targets to develop preventive and therapeutic approaches against enterococci.

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Reference66 articles.

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