Generation and Functional Analysis of Defective Viral Genomes during SARS-CoV-2 Infection

Author:

Zhou Terry1,Gilliam Nora J.123,Li Sizhen4,Spandau Simone1,Osborn Raven M.13,Connor Sarah5,Anderson Christopher S.5ORCID,Mariani Thomas J.5,Thakar Juilee167,Dewhurst Stephen1,Mathews David H.8,Huang Liang4,Sun Yan1ORCID

Affiliation:

1. Department of Immunology and Microbiology, University of Rochester Medical Center, Rochester, New York, USA

2. Medical Scientist Training Program, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA

3. Translational Biomedical Sciences PhD Program, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA

4. School of Electrical Engineering & Computer Science, Oregon State University, Corvallis, Oregon, USA

5. Department of Pediatrics and Center for Children’s Health Research, University of Rochester, Rochester, New York, USA

6. Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA

7. Department of Biomedical Genetics, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA

8. Department of Biochemistry & Biophysics and Center for RNA Biology, University of Rochester Medical Center, Rochester, New York, USA

Abstract

Defective viral genomes (DVGs) are generated ubiquitously in many RNA viruses, including SARS-CoV-2. Their interference activity to full-length viruses and IFN stimulation provide the potential for them to be used in novel antiviral therapies and vaccine development.

Funder

University of Rochester Technology Development Fund

University of Rochester HSCCI

Burroughs Wellcome Fund

HHS | NIH | National Center for Advancing Translational Sciences

HHS | NIH | National Heart, Lung, and Blood Institute

HHS | NIH | National Institute of General Medical Sciences

UR | School of Medicine and Dentistry, University of Rochester

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

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