Affiliation:
1. Institut für Allgemeine Mikrobiologie der Universität, Bern, Switzerland
Abstract
Megnet, Roland
(Institut für Allgemeine Mikrobiologie der Universität, Bern, Switzerland). Effect of 2-deoxyglucose on
Schizosaccharomyces pombe
. J. Bacteriol.
90:
1032–1035. 1965.—Cultivation of
Schizosaccaromyces pombe
in a medium containing 2-deoxyglucose (100 μg/ml) results in the death of the cells after an initial period of apparently normal growth. At higher deoxyglucose concentration (400 μg/ml), the cells die immediately after inoculation. Only growing cells are killed, and microscopic inspection of the cultures reveals cell-wall fragments of lysed cells. A mutant resistant to 2-deoxyglucose, which cannot use glucose as a carbon source, was found to be partially deficient in hexokinase. The data constitute evidence for the inhibition of some reaction(s) in the synthesis of cell-wall polysaccharides by metabolites of 2-deoxyglucose in this organism.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference11 articles.
1. CRANE R. K. 1962. Hexokinase and pentokinases p. 47-66. In P. D. Boyer H. Lardy and K. Myrback [ed.] The enzymes vol. 6. Academic Press Inc. New York.
2. The substrate specificities of phosphoglucomutase and phosphoglucose isomerase;EGYUJD L. G.;Biochem. J.,1963
3. Sugar nucleotides and the synthesis of carbohydrates;GINSBURG V.;Advan. Enzymol.,1964
4. Metabolic studies with 2-deoxyhexoses;HEREDIA C. F.;Biochim. Biophys. Acta,1964
5. LAYNE E. 1955. Spectrophotometric and turbidimetric methods for measuring proteins p. 447-454. In S. P. Colowick and N. 0. Kaplan [ed.] Methods in enzymology vol. 3. Academic Press Inc. New York.
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