Effect of 2-Deoxyglucose on Schizosaccharomyces pombe

Author:

Megnet Roland1

Affiliation:

1. Institut für Allgemeine Mikrobiologie der Universität, Bern, Switzerland

Abstract

Megnet, Roland (Institut für Allgemeine Mikrobiologie der Universität, Bern, Switzerland). Effect of 2-deoxyglucose on Schizosaccharomyces pombe . J. Bacteriol. 90: 1032–1035. 1965.—Cultivation of Schizosaccaromyces pombe in a medium containing 2-deoxyglucose (100 μg/ml) results in the death of the cells after an initial period of apparently normal growth. At higher deoxyglucose concentration (400 μg/ml), the cells die immediately after inoculation. Only growing cells are killed, and microscopic inspection of the cultures reveals cell-wall fragments of lysed cells. A mutant resistant to 2-deoxyglucose, which cannot use glucose as a carbon source, was found to be partially deficient in hexokinase. The data constitute evidence for the inhibition of some reaction(s) in the synthesis of cell-wall polysaccharides by metabolites of 2-deoxyglucose in this organism.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference11 articles.

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5. LAYNE E. 1955. Spectrophotometric and turbidimetric methods for measuring proteins p. 447-454. In S. P. Colowick and N. 0. Kaplan [ed.] Methods in enzymology vol. 3. Academic Press Inc. New York.

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