Reduction of nonspecific fluorescence in respiratory specimens by pretreatment with N-acetylcysteine

Author:

Miller H R,Phipps P H,Rossier E

Abstract

Treatment of nasopharyngeal specimens with 0.25% N-acetylcysteine for 20 min at room temperature effectively reduced the nonspecific fluorescence encountered during direct examination of the specimens by immunofluorescence. The treatment did not affect specific antigen staining or the viability of several common respiratory viruses.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference5 articles.

1. Laboratory and clinical evaluation of the mucolytic properties of acetylcysteine;Hurst G. A.;Am. Rev. Respir. Dis.,1967

2. Sputum digestion and decontamination with N-acetylcysteine-sodium hydroxide for culture of mycobacteria;Kubica G. P.;Am. Rev. Respir. Dis.,1963

3. Enzymatic Iysis of respiratory secretions by aerosol trypsin;Limber C. R.;J. A. Med. Assoc.,1952

4. Use of dithiothreitol to replace N-acetyl-L-cysteine for routine sputum digestion-decontamination for the culture of mycobacteria;Shah R. R.;Am. Rev. Respir. Dis.,1966

5. The reduction in vitro in viscosity of mucoprotein solutions by a new mucolytic agent, N-acetyl-Lcysteine;Sheffner A. L.;Ann. N. Y. Acad. Sci.,1963

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