Development of a TaqMan Multiplex PCR Assay for Detection of Plasmid-Mediated AmpC β-Lactamase Genes-Reference-Cited by-同舟云学术

Development of a TaqMan Multiplex PCR Assay for Detection of Plasmid-Mediated AmpC β-Lactamase Genes

Published:2012-11 Issue:11 Volume:50 Page:3722-3725
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Geyer Chelsie N.¹,Reisbig Mark D.¹,Hanson Nancy D.¹

Affiliation:

1. Creighton University School of Medicine Department of Medical Microbiology and Immunology Center for Research in Anti-Infectives and Biotechnology (C.R.A.B), Omaha, Nebraska, USA

Abstract

ABSTRACT A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02038-12

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