Development and Evaluation of a Novel Multiplex PCR Technology for Molecular Differential Detection of Bacterial Respiratory Disease Pathogens

Author:

Benson Robert1,Tondella Maria L.1,Bhatnagar Julu1,Carvalho Maria da Glória S.1,Sampson Jacquelyn S.1,Talkington Deborah F.1,Whitney Anne M.1,Mothershed Elizabeth1,McGee Lesley2,Carlone George1,McClee Vondguraus1,Guarner Jeannette12,Zaki Sherif1,Dejsiri Surang3,Cronin K.4,Han Jian4,Fields Barry S.1

Affiliation:

1. CDC, Atlanta, Georgia

2. Emory University, Atlanta, Georgia

3. National Institute of Health, Thailand Ministry of Public Health, Bangkok, Thailand

4. Genaco Biomedical Products, Inc., Huntsville, Alabama

Abstract

ABSTRACT The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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