Development and Evaluation of a Novel Multiplex PCR Technology for Molecular Differential Detection of Bacterial Respiratory Disease Pathogens-Reference-Cited by-同舟云学术

Development and Evaluation of a Novel Multiplex PCR Technology for Molecular Differential Detection of Bacterial Respiratory Disease Pathogens

Published:2008-06 Issue:6 Volume:46 Page:2074-2077
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Benson Robert¹,Tondella Maria L.¹,Bhatnagar Julu¹,Carvalho Maria da Glória S.¹,Sampson Jacquelyn S.¹,Talkington Deborah F.¹,Whitney Anne M.¹,Mothershed Elizabeth¹,McGee Lesley²,Carlone George¹,McClee Vondguraus¹,Guarner Jeannette¹²,Zaki Sherif¹,Dejsiri Surang³,Cronin K.⁴,Han Jian⁴,Fields Barry S.¹

Affiliation:

1. CDC, Atlanta, Georgia

2. Emory University, Atlanta, Georgia

3. National Institute of Health, Thailand Ministry of Public Health, Bangkok, Thailand

4. Genaco Biomedical Products, Inc., Huntsville, Alabama

Abstract

ABSTRACT The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01858-07

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