Molecular Diagnosis of Human Rhinovirus Infections: Comparison with Virus Isolation-Reference-Cited by-同舟云学术

Molecular Diagnosis of Human Rhinovirus Infections: Comparison with Virus Isolation

Published:1998-07 Issue:7 Volume:36 Page:2081-2083
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hyypiä Timo¹,Puhakka Tuomo²,Ruuskanen Olli²,Mäkelä Mika²,Arola Anita¹²,Arstila Pertti¹

Affiliation:

1. Departments of Virology1 and

2. Pediatrics,2 University of Turku, FIN-20520 Turku, Finland

Abstract

ABSTRACT To compare the sensitivity and specificity of RT-PCR with that of virus isolation in the detection of human rhinoviruses, we tested nasopharyngeal aspirates from 200 patients on the 1st and 7th days after the onset of the common cold. An assay utilizing a short amplicon in the conserved 5′ noncoding region was found highly sensitive. Of 192 positive samples altogether, 65 were found positive by RT-PCR only, 6 were positive by isolation exclusively, and 121 gave positive results in both tests.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.7.2081-2083.1998

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