Affiliation:
1. Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
Abstract
Some ribosomal proteins have extraribosomal functions in addition to ribosome translation function. The extraribosomal functions of several r-proteins control operon expression by binding to own-operon transcripts. Previously, we discovered a posttranscriptional, RNase E-dependent regulatory role for r-protein L4 in the stabilization of stress-responsive transcripts. Here, we found an additional extraribosomal function for L4 in regulating the
tna
operon by L4-intergenic spacer mRNA interactions. L4 binds to the transcribed spacer RNA between
tnaC
and
tnaA
and alters the structural conformation of the spacer RNA, thereby reducing the translation of TnaA. Our study establishes a previously unknown L4-mediated mechanism for regulating gene expression, suggesting that bacterial cells have multiple strategies for controlling levels of tryptophanase in response to varied cell growth conditions.
Funder
Ministry of Science and Technology, Taiwan
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
4 articles.
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