Affiliation:
1. Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University, Tsushima-Naka 3-1-1, Okayama 700-8530, Japan
Abstract
ABSTRACT
Flagellar operons are divided into three classes with respect to their transcriptional hierarchy in
Salmonella enterica
serovar Typhimurium. The class 1 gene products FlhD and FlhC act together in an FlhD
2
C
2
heterotetramer, which binds upstream of the class 2 promoters to facilitate binding of RNA polymerase. Class 2 expression is known to be enhanced by a disruption mutation in a flagellar gene,
fliT
. In this study, we purified FliT protein in a His-tagged form and showed that the protein prevented binding of FlhD
2
C
2
to the class 2 promoter and inhibited FlhD
2
C
2
-dependent transcription. Pull-down and far-Western blotting analyses revealed that the FliT protein was capable of binding to FlhD
2
C
2
and FlhC and not to FlhD alone. We conclude that FliT acts as an anti-FlhD
2
C
2
factor, which binds to FlhD
2
C
2
through interaction with the FlhC subunit and inhibits its binding to the class 2 promoter.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
80 articles.
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