Affiliation:
1. Department of Internal Medicine, Division of Infectious Diseases, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267
Abstract
ABSTRACT
Candida albicans
is a component of the normal flora of the alimentary tract and also is found on the mucocutaneous membranes of the healthy host.
Candida
is the leading cause of invasive fungal disease in premature infants, diabetics, and surgical patients, and of oropharyngeal disease in AIDS patients. As the induction of cell-mediated immunity to
Candida
is of critical importance in host defense, we sought to determine whether human dendritic cells (DC) could phagocytose and degrade
Candida
and subsequently present
Candida
antigens to T cells. Immature DC obtained by culture of human monocytes in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4 phagocytosed unopsonized
Candida
in a time-dependent manner, and phagocytosis was not enhanced by opsonization of
Candida
in serum. Like macrophages (Mφ), DC recognized
Candida
by the mannose-fucose receptor. Upon ingestion, DC killed
Candida
as efficiently as human Mφ, and fungicidal activity was not enhanced by the presence of fresh serum. Although phagocytosis of
Candida
by DC stimulated the production of superoxide anion, inhibitors of the respiratory burst (or NO production) did not inhibit killing of
Candida
, even when phagocytosis was blocked by preincubation of DC with cytochalasin D. Further, although apparently only modest phagolysosomal fusion occurred upon DC phagocytosis of
Candida
, killing of
Candida
under anaerobic conditions was almost equivalent to killing under aerobic conditions. Finally, DC stimulated
Candida
-specific lymphocyte proliferation in a concentration-dependent manner after phagocytosis of both viable and heat-killed
Candida
cells. These data suggest that, in vivo, such interactions between DC and
C. albicans
may facilitate the induction of cell-mediated immunity.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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