Optochin Revisited: Defining the Optimal Type of Blood Agar for Presumptive Identification of Streptococcus pneumoniae

Author:

Gardam M. A.1,Miller M. A.1

Affiliation:

1. Sir Mortimer B. Davis-Jewish General Hospital, McGill University, Montreal, Canada

Abstract

ABSTRACT To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae , we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia agar, Trypticase soy agar (TSA), and Mueller-Hinton agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood agar be used.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference6 articles.

1. Importance of Carbon Dioxide in the Isolation of Pneumococci

2. The optochin sensitivity test: a reliable method for identification of pneumococci;Bowen M. K.;J. Lab. Clin. Med.,1957

3. Optochin in the identification of Str. pneumoniae;Bowers E. F.;J. Clin. Pathol.,1955

4. Isenberg H. D. Clinical microbiology procedures handbook 1992 1.20.25 1.20.26 American Society for Microbiology Washington D.C

5. The action of ethylhydrocupreine (optochin) on type strains of pneumococci in vitro and in vivo, and on some other microorganisms in vitro;Moore H. F.;J. Exp. Med.,1915

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