PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells-Reference-Cited by-同舟云学术

PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells

Published:2001-05 Issue:9 Volume:75 Page:4407-4412
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Johnson Marc C.¹,Scobie Heather M.¹,Vogt Volker M.¹

Affiliation:

1. Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853

Abstract

ABSTRACT While baculovirus expression of Gag proteins from numerous retroviruses has led reliably to production of virus-like particles (VLPs), we observed that expression of Rous sarcoma virus Gag failed to produce VLPs. Transmission and scanning electron microscopy analysis revealed that the Gag protein reached the plasma membrane but was unable to correctly form particles. Addition of a myristylation signal had no effect on the budding defect, but deletion of the PR domain of Gag restored normal budding. The resulting VLPs were morphologically distinct from human immunodeficiency virus type 1 VLPs expressed in parallel.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.75.9.4407-4412.2001

Reference32 articles.

1. Differential budding efficiencies of human T-cell leukemia virus type I (HTLV-I) Gag and Gag-Pro polyproteins from insect and mammalian cells;Bouamr F.;Virology,2000

2. In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles

3. The GAG precursor of simian immunodeficiency virus assembles into virus-like particles;Delchambre M.;EMBO J.,1989

4. Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle;Fuller S. D.;Curr. Biol.,1997

5. Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells;Gheysen D.;Cell,1989

Cited by 19 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Dual display hemagglutinin 1 and 5 on the surface of enveloped virus-like particles in silkworm expression system;Protein Expression and Purification;2022-09

2. Distinct Particle Morphologies Revealed through Comparative Parallel Analyses of Retrovirus-Like Particles;Journal of Virology;2016-09-15

3. Hydrodynamic and Membrane Binding Properties of Purified Rous Sarcoma Virus Gag Protein;Journal of Virology;2015-10-15

4. A Large Extension to HIV-1 Gag, Like Pol, Has Negative Impacts on Virion Assembly;PLoS ONE;2012-10-23

5. In Vitro Assembly of Virus-Like Particles of a Gammaretrovirus, the Murine Leukemia Virus XMRV;Journal of Virology;2011-11-16