Evaluation of Quantitative PCR and Branched-Chain DNA Assay for Detection of Hepatitis B Virus DNA in Sera from Hepatocellular Carcinoma and Liver Transplant Patients

Author:

Chen Tao1,Luk John M.1,Cheung Siu-Tim1,Yu Wan-Ching1,Fan Sheung-Tat1

Affiliation:

1. Center of Liver Diseases and Department of Surgery, University of Hong Kong Medical Center, Queen Mary Hospital, Pokfulam, Hong Kong

Abstract

ABSTRACT This study evaluated the applicability of quantitative PCR (Q-PCR) and branched-chain DNA assays for detection of hepatitis B virus (HBV) DNA in sera. For 42 samples, the detection rates were 81 and 41%, respectively, with a correlation coefficient of 0.633. The Q-PCR is useful for early monitoring of HBV load in high-risk patients.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference24 articles.

1. Review: hepatitis B and liver transplantation;Angus P. W.;J. Gastroenterol. Hepatol.,1997

2. Hepatitis B virus. The major etiology of hepatocellular carcinoma;Beasley R. P.;Cancer,1988

3. Mini-pool screening by nucleic acid testing for hepatitis B virus, hepatitis C virus, and HIV: preliminary results;Cardoso M. S.;Transfusion,1998

4. Hepatitis B virus replication status and its response to surgery for tumor in hepatocellular carcinoma with positive markers of HBV;Chen T.;Chin. J. Surgery,1998

5. Comparative evaluation of hepatitis C virus RNA quantitation by branched DNA, NASBA, and Monitor assays;Francoise L.;Hepatology,1999

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3