Multiplex PCRs for Identification of Escherichia coli Virulence Genes-Reference-Cited by-同舟云学术

Multiplex PCRs for Identification of Escherichia coli Virulence Genes

Published:2000-05 Issue:5 Volume:38 Page:2001-2004
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pass M. A.¹,Odedra R.²,Batt R. M.²

Affiliation:

1. Department of Physiology and Pharmacology, The University of Queensland, St. Lucia, Queensland, 4072, Australia,1 and

2. Department of Small Animal Medicine and Surgery, The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, AL9 7TA, United Kingdom2

Abstract

ABSTRACT PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.5.2001-2004.2000

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