Identification of a Secreted Cholesterol-Dependent Cytolysin (Mitilysin) from Streptococcus mitis-Reference-Cited by-同舟云学术

Identification of a Secreted Cholesterol-Dependent Cytolysin (Mitilysin) from Streptococcus mitis

Published:2007-01-15 Issue:2 Volume:189 Page:627-632
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Jefferies Johanna¹,Nieminen Leena¹,Kirkham Lea-Ann¹,Johnston Calum¹,Smith Andrew²,Mitchell Tim J.¹

Affiliation:

1. Division of Infection and Immunity, Glasgow Biomedical Research Centre, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8TA

2. Infection Research Group, Level 9, Glasgow Dental Hospital, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom

Abstract

ABSTRACT We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis . Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mitilysin extracellularly. Certain alleles of mitilysin are not recognized by a monoclonal antibody raised to the related toxin pneumolysin. Based on enzyme-linked immunosorbent assay and neutralization assay results, one isolate of S. mitis may produce a further hemolytic toxin in addition to mitilysin. As genetic exchange is known to occur between S. mitis and Streptococcus pneumoniae , this finding may have implications for the development of vaccines or therapies for pneumococcal disease that are based on pneumolysin.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.01092-06

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