Affiliation:
1. Department of Laboratory Medicine, Kosin University College of Medicine, Busan 602-702
2. Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea
3. Department of Biological Science, Myongji University, Yongin, Kyunggido 449-728
4. Bio Technology Innovation Center, Youngdong University, Chungbuk 370-701
Abstract
ABSTRACT
To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of
Klebsiella pneumoniae
and
Escherichia coli
, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producing isolates were detected in every region in Korea. A total of 44.6% of the isolates produced both TEM- and SHV-type ESBLs, and 52% of ESBL-producing isolates transferred resistance to ceftazidime by transconjugation. The ESBLs were TEM-19, TEM-20, TEM-52, SHV-2a, SHV-12, and one new variant identified for the first time in Korea, namely, TEM-116. TEM-1 and SHV-12 were by far the most common variants. TEM-1, TEM-116, and SHV-12 showed a high prevalence in
K. pneumoniae
. Two isolates (
E. coli
SH16 and
K. pneumoniae
SV3) produced CMY-1-like beta-lactamases, which play a decisive role in resistance to cefoxitin and cefotetan, as well as TEM-type enzymes (TEM-20 and TEM-52, respectively). Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52.
Publisher
American Society for Microbiology
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