Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods-Reference-Cited by-同舟云学术

Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods

Published:2004-07 Issue:7 Volume:42 Page:3333-3335
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Procop G. W.¹,Haddad S.²,Quinn J.³,Wilson M. L.³,Henshaw N. G.⁴⁵,Reller L. B.⁴⁵⁶,Artymyshyn R. L.²,Katanik M. T.¹,Weinstein M. P.⁷⁸²

Affiliation:

1. Section of Clinical Microbiology, Department of Clinical Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio

2. Pathology, University of MedicineDentistry—Robert Wood Johnson Medical School, New Brunswick, New Jersey

3. Department of Pathology, Denver Health Medical Center, Denver, Colorado

4. Clinical Microbiology Laboratory

5. Departments of Pathology

6. Medicine, Duke University Medical Center, Durham, North Carolina

7. Microbiology Laboratory

8. Departments of Medicine

Abstract

ABSTRACT We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain were 49.2 and 99.6%, respectively. The sensitivity and specificity of Merifluor Pneumocystis stain were 90.8 and 81.9%, respectively. Only CW and GMS had positive and negative predictive values of >90%.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.7.3333-3335.2004

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