A Novel Multiplex Real-Time PCR Assay for Rapid Typing of Major Staphylococcal Cassette Chromosome mec Elements-Reference-Cited by-同舟云学术

A Novel Multiplex Real-Time PCR Assay for Rapid Typing of Major Staphylococcal Cassette Chromosome mec Elements

Published:2004-07 Issue:7 Volume:42 Page:3309-3312
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Francois Patrice¹,Renzi Gesuele²,Pittet Didier³,Bento Manuela¹,Lew Daniel¹²,Harbarth Stephan³,Vaudaux Pierre¹,Schrenzel Jacques¹²

Affiliation:

1. Genomic Research Laboratory

2. Clinical Microbiology Laboratory, Division of Infectious Diseases

3. Infection Control Program, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland

Abstract

ABSTRACT We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.7.3309-3312.2004

Reference21 articles.

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