Complete reference genomes of two ceftriaxone-resistant <i>Neisseria gonorrhoeae</i> strains identified in routine surveillance in Bangkok, Thailand, using Nanopore Q20+ chemistry, VolTRAX V2b, and Illumina sequencing-Reference-Cited by-同舟云学术

Complete reference genomes of two ceftriaxone-resistant Neisseria gonorrhoeae strains identified in routine surveillance in Bangkok, Thailand, using Nanopore Q20+ chemistry, VolTRAX V2b, and Illumina sequencing

Published:2024-02-02 Issue: Volume: Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Sangprasert Pongsathorn¹,Golparian Daniel²,Paopang Porntip¹,Girdthep Natnaree¹,Lawung Ratana³,Gopinath Deyer⁴,Thammawijaya Panithee⁵,Kittiyaowanarn Rossaphorn¹,Unemo Magnus²⁶^ORCID

Affiliation:

1. Division of AIDS and STIs, Department of Disease Control and Prevention, Bangrak STIs Center, Thailand Ministry of Public Health, Bangkok, Thailand

2. Department of Laboratory Medicine, Microbiology, Faculty of Medicine and Health, World Health Organization (WHO) Collaborating Centre for Gonorrhoea and Other Sexually Transmitted Infections, Örebro University, Örebro, Sweden

3. Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand

4. World Health Organization (WHO), Country Office, Nonthaburi, Thailand

5. Department of Disease Control, Bangkok, Thailand

6. Institute for Global Health, University College London (UCL), London, United Kingdom

Abstract

ABSTRACT Ceftriaxone-resistant Neisseria gonorrhoeae strains, mostly associated with Asia, threaten gonorrhea treatment. We report the reference genomes of two ceftriaxone-resistant isolates found in routine surveillance in Bangkok, Thailand. The genomes belonged to the more antimicrobial-susceptible genomic lineage B, illustrating that both ceftriaxone-resistant strains and the mosaic penA -60.001 ceftriaxone-resistance determinant are spreading.

Funder

Orebro County Council Research Committee

Foundation for Medical Research at Orebro University Hospital

Bangrak STIs Center

WHO Thailand

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/mra.01231-23

Reference10 articles.

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4. Genomic evolution of Neisseria gonorrhoeae since the preantibiotic era (1928–2013): antimicrobial use/misuse selects for resistance and drives evolution

5. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads