Influence of Osmotic and Nutritional Stress on Physiology of Penicillium fellutanum in Removal of Phosphocholine and Modification of Phospho-1- O -[ N -Peptidyl-(2-Aminoethanol)] Phosphodiesters of Peptidophosphogalactomannan Species

Abstract

ABSTRACT Addition of 3 M NaCl to 72-h cultures of Penicillium fellutanum in 2 mM phosphate resulted in an increase in percentage of extracellular peptidophosphogalactomannan III (pP x GM iii ) and a decrease in that of pP x GM ii . The magnitude of 31 P nuclear magnetic resonance signals at 1.47 and 1.33 ppm of phospho-1- O -[ N -peptidyl-(2-aminoethanol)] phosphodiesters pP x GM ii and pP x GM iii decreased compared with controls. The data suggest that serine, glycine, and threonine residues from the 3-kDa peptide and from galactofuranosyl-6- O -phospho-1′- O -[ N -peptidyl-(2-aminoethanol)] residues were the precursors of the needed choline-derived osmolytes.