Characterization of Extracellular Vesicles from Entamoeba histolytica Identifies Roles in Intercellular Communication That Regulates Parasite Growth and Development

Author:

Sharma Manu1,Morgado Pedro1,Zhang Hanbang1,Ehrenkaufer Gretchen1,Manna Dipak1,Singh Upinder12ORCID

Affiliation:

1. Division of Infectious Diseases, Stanford University School of Medicine, Stanford, California, USA

2. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA

Abstract

Extracellular vesicles (EVs) secreted by eukaryotic and prokaryotic cells to transport lipids, proteins, and nucleic acids to the external environment have important roles in cell-cell communication through cargo transfer. We identified and characterized EVs from Entamoeba histolytica , a protozoan parasite and a human pathogen. Conditioned medium from amebic parasites contained particles consistent with the expected size and morphology of EVs. Mass spectrometry was used to characterize the EV proteome and showed that it was enriched in common exosome marker proteins, including proteins associated with vesicle formation, cell signaling, and metabolism, as well as cytoskeletal proteins.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

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