Affiliation:
1. Division of Communicable Disease and Immunology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C.
Abstract
Falkow, Stanley
(Walter Reed Army Institute of Research, Washington, D.C.), H.
Schneider, L. S. Baron, and S. B. Formal
. Virulence of
Escherichia-Shigella
hybrids for the guinea pig. J. Bacteriol.
86:
1251–1258. 1963.—Genetic recombination studies between donor
Escherichia coli
and recipient
Shigella flexneri
2a strains were employed to examine alterations in the virulence of
Shigella
hybrids for guinea pigs. The genetic studies indicated that several chromosomal regions of
E. coli
and
S. flexneri
are grossly homologous. The frequency of recombination between
E. coli
and
Shigella
was decreased, however, in comparison with
E. coli
×
E. coli
matings. Moreover, the predominant
Shigella
hybrid classes acquired only the selected genetic marker, and extensive transfer of the
Escherichia
genome was detected only occasionally. The virulence studies made use of hybrids with well-defined single markers as well as those with overlapping chromosomal regions. Analysis of over one-half of the chromosome revealed only one chromosomal region, located between the
rha
+
and
xyl
+
genes, which was essential for virulence. However, hybrids which had received the
E. coli
pili antigen and
fuc
+
-
nic
+
determinants exhibited an intermediate virulence. Hybrids carrying a full complement of
Shigella
genes and the
rha
+
-
xyl
+
Escherichia
region as a persistent exogenote (partial diploids) were of intermediate virulence. These partial diploids may return to complete virulence by elimination of the
Escherichia
chromosomal fragment or become avirulent by incorporation of this fragment.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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