Rapid Approach To Determine rrn Arrangement in Salmonella Serovars

Author:

Helm R. Allen1,Maloy Stanley12

Affiliation:

1. Department of Microbiology, University of Illinois, Urbana, Illinois,1 and

2. Center for Microbial Sciences, San Diego, California2

Abstract

ABSTRACT A PCR method was developed by which to rapidly and accurately determine the rrn arrangement of Salmonella enterica serovars. Primers were designed to the genomic regions flanking each of the seven rrn operons . PCR analysis using combinations of these primers will distinguish each of the possible arrangements of the rrn skeleton.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference14 articles.

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3. Sequence variation of the 16S to 23S rRNA spacer region in Salmonella enterica.;Christensen H.;Res. Microbiol.,2000

4. Chromosomal locations of the genes for rRNA in Escherichia coli K-12.

5. Hill C. W. Harvey S. Gray J. A. (1990) Recombination between rRNA genes in Escherichia coli and Salmonella typhimurium . in The bacterial chromosome. eds DrlicaK.RileyM. (American Society for Microbiology Washington D.C.) pp 335–340.

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