New Antifungal Susceptibility Test Based on Chitin Detection by Image Cytometry-Reference-Cited by-同舟云学术

New Antifungal Susceptibility Test Based on Chitin Detection by Image Cytometry

Published:2019-12-20 Issue:1 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Wang Yan¹,Andriampamonjy Arisandy N.¹,Bailly Sebastien²,Garnaud Cécile¹,Maubon Danièle¹,Cornet Muriel¹^ORCID,Aldebert Delphine¹^ORCID

Affiliation:

1. University Grenoble Alpes, CNRS, Grenoble INP, CHU Grenoble Alpes, TIMC-IMAG, Grenoble, France

2. University Grenoble Alpes, INSERM, CHU Grenoble Alpes, HP2, Thorax and vessels, EFCR, Grenoble, France

Abstract

The antifungal susceptibility tests used in clinical laboratories have several limitations. We developed a new test, SensiFONG, based on the detection of chitin levels after exposure to antifungal drugs. The optimal culture conditions were 30°C for 6 h for yeast strains and 26°C for 16 h for molds. The strains were exposed to a range of echinocandin or azole concentrations. Chitin was stained with calcofluor white.

Funder

SATT Linksium

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01101-19

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