Quinolone Resistance in Neisseria gonorrhoeae : Rapid Genotyping of Quinolone Resistance-Determining Regions in gyrA and parC Genes by Melting Curve Analysis Predicts Susceptibility-Reference-Cited by-同舟云学术

Quinolone Resistance in Neisseria gonorrhoeae : Rapid Genotyping of Quinolone Resistance-Determining Regions in gyrA and parC Genes by Melting Curve Analysis Predicts Susceptibility

Published:2009-03 Issue:3 Volume:53 Page:1264-1267
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Vernel-Pauillac Frédérique¹,Hogan Tiffany R.²,Tapsall John W.²,Goarant Cyrille¹

Affiliation:

1. Institut Pasteur de Nouvelle-Calédonie, Laboratoire de Recherche en Bactériologie, Nouméa Cedex, New Caledonia

2. WHO Collaborating Centre for STD, Department of Microbiology, The Prince of Wales Hospital, Sydney, Australia

Abstract

ABSTRACT We report a duplex real-time PCR assay for the simultaneous screening of mutations involved in fluoroquinolone resistance within gyrA and parC quninolone resistance-determining regions (QRDRs) in Neisseria gonorrhoeae . Our assay clearly detects all mutated QRDRs and allows the identification of common genotypes, whether the QRDRs contain single or double mutations, providing valuable epidemiological tools. When this method is used in conjunction with similar assays and in vitro analyses, essential antibiotic resistance surveillance can be performed for public health purposes.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01104-08

Reference23 articles.

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