Affiliation:
1. Department of Microbiology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642
Abstract
Whole cells of
Bacillus subtilis
transported
d
-alanine and
l
-alanine by two different systems. The high-affinity system (
K
m
of 1 μM and
V
max
of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (
K
m
of 10 μM for
l
-alanine and 20 μM for
d
-alanine and glycine) had a
V
max
of 5 to 12 nmol/min per mg of protein. This system transported glycine,
d
-cycloserine, and
d
-serine, in addition to
d
- and
l
-alanine. Azide inhibited the uptake of these amino acids and caused the efflux of
d
-alanine from preloaded cells. These data suggest that transport of these amino acids is energized by the electron transport chain.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
10 articles.
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