Polymerization of the Actin-Like Protein MamK, Which Is Associated with Magnetosomes

Author:

Taoka Azuma1,Asada Ryuji1,Wu Long-Fei2,Fukumori Yoshihiro1

Affiliation:

1. Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan

2. Laboratoire de Chimie Bactérienne, UPR9043, IBSM, CNRS, 31, Chemin Joseph Aiguier, 13402 Marseille cedex 20, France

Abstract

ABSTRACT The recombinant actin-like protein MamK was purified from Escherichia coli and used as an antigen to generate the anti-MamK antibody. Immunostaining studies showed a linear distribution of MamK in Magnetospirillum magnetotacticum cells and of MamK in association with magnetosomes. Moreover, we demonstrated that MamK polymerizes into filamentous bundles in vitro.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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