Affiliation:
1. Department of Microbiology and Enzymology, Kluyver Laboratory for Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
Abstract
Nuclepore polycarbonate filters floating on a liquid, FeSO
4
-containing medium (pH 1.6) were used to isolate a moderately thermophilic bacterium from a pyrite-oxidizing enrichment culture. The isolate failed to grow on any of the conventional solid media tried. To test the general applicability of the method, the enumeration of a fastidious acidophilic organism,
Thiobacillus ferrooxidans
, was carried out and the results compared with those obtained with other filters, solid media, and the most probable number technique.
T. ferrooxidans
showed better viability on the floating polycarbonate filters and grew in a much shorter time (4 to 5 days) than with the other techniques.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference9 articles.
1. Boogerd F. C. M. M. Q. van Alphen W. J. van Anrooij J. C. de Bruyn P. Bos and J. G. Kuenen. 1990. The role of growth and maintenance in the oxidation of pyrite in batch culture by a moderately thermophilic facultative chemolithautotroph p. 735-751. In J. Salley R. G. L. McCready and P. L. Wichlacz (ed.) Proceedings of the 1989 Intemational Symposium on Biohydrometallurgy. Canada Centre for Mineral and Energy Technology Ottawa Ontario Canada.
2. Growth of Thiobacillus ferrooxidans on solid media containing heterotrophic bacteria;Butler B. J.;J. Ind. Microbiol.,1987
3. An iron-oxidizing bacterium from the acid drainage of some bituminous coal mines;Colmer A. R.;J. Bacteriol.,1950
4. The acidophilic Thiobacilli and other acidophilic bacteria that share their habitat. Annu;Harrison A. P.;Rev. Microbiol.,1984
5. Use of Nuclepore filters for counting bacteria by fluorescence microscopy;Hobbie J. E.;Appl. Environ. Microbiol.,1977
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