Abstract
This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data confirming this relationship are given. Alkylating agents and mycotoxins were used to test the system. This method can be used to follow up decreased mutagenic potencies of solutions of unstable mutagens and to follow the production of mutagenic substances throughout fermentation.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference11 articles.
1. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test;Ames B. N.;Mutat. Res.,1975
2. A quantitative model for diffusion bioassays;Awerbuch T. E.;J. Theor. Biol.,1979
3. Short term screening tests for carcinogens;Bridges B. A.;Nature (London),1976
4. Cooper K. E. 1963. The theory of antibiotic inhibition zones p. 1-86. In F. Kavanaugh (ed.) Analytical microbiology. Academic Press Inc. New York.
5. Ghosh A. C. A. Manmade and A. L. Demain. 1977. Toxins from P. islandicum Sopp. p. 625-638. In J. V. Rodnicks C. W. Hesseltine and M. A. Mehlman (ed.) Mycotoxins in human and animal health. Pathotox Publishers Park Forest 111.
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