Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis

Author:

Evans R T,Taylor-Robinson D

Abstract

McCoy cells treated in six different ways, in addition to untreated cells, were compared to determine which gave rise to the largest number of Chlamydia trachomatis inclusions when tested with a laboratory-passaged strain. The same batch of cells was treated by irradiation, preinoculation exposure to cytochalasin B or 5-iodo-2'-deoxyuridine, and postinoculation exposure to cycloheximide, hydrocortisone, or emetine. Significantly more inclusions were always found in cells which had been treated with cycloheximide than in cells treated in any other way. Conversely, untreated McCoy cells always had significantly fewer inclusions than cells which had received some form of treatment. Similar results were obtained when cycloheximide-treated, irradiated, and untreated cells were inoculated with urethral specimens containing unpassaged organisms.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference19 articles.

1. Synthesis of trachoma agent in emetine-treated cells;Becker Y.;J. Bacteriol.,1972

2. Cultivation of TRIC agents: a comparison between the use of BHK21 and irradiated McCoy cells;Blythe W. A.;J. Hyg.,1974

3. Effect of cortisol on the growth of Chiamydia trachomatis in McCoy cells;Bushell A. C.;Infect. Immun.,1978

4. Comparative susceptibility of eleven mammalian cell lines to infection with trachoma organisms;Croy T. R.;J. Clin. Microbiol.,1975

5. Chlamydia trachomatis from men with non-gonococcal urethritis. Simplified procedure for cultivation and isolation in replicating McCoy cell culture;Csango P.A.;Acta Pathol. Microbiol. Scand. Sect. B,1978

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