Affiliation:
1. Louisiana State University Medical Center, New Orleans 70112.
Abstract
The susceptibilities of 36 isolates of the Bacteroides fragilis group to ceftizoxime, cefoxitin, clindamycin, and metronidazole were determined by using the National Committee for Clinical Laboratory Standards agar dilution reference method and a broth microdilution method using anaerobe, brucella, Schaedler, and brain heart infusion broths. MICs that were greater than or equal to fourfold higher or lower than those of the reference method were considered significant. Major and minor discrepancies in susceptibility interpretation (SI) were also noted. Ceftizoxime showed the greatest number of variations and SI discrepancies. In 72% of the cases, MICs in broth were greater than or equal to fourfold lower than those obtained by the reference method, resulting in 33% of the major and 22% of the minor discrepancies in SI. A total of 53% of the isolates were resistant to ceftizoxime by the reference method, but only 11 to 17% were resistant in the various broths. Significant variations in MICs of cefoxitin occurred in 19 to 22% of the isolates; 17 to 19% of the isolates showed major discrepancies and 31 to 58% showed minor discrepancies in SI. A total of 58% of the isolates were resistant to cefoxitin by the reference method, but only 19 to 28% were resistant in the various broths. Significant variations with clindamycin in broths ranged from 32 to 53% and resulted in 3 to 8% of the isolates showing major discrepancies and 33 to 44% showing minor discrepancies in SI. Metronidazole yielded significant variations in MICs in 6 to 28% of the isolates, but no major or minor SI discrepancies were noted. This study indicates that significant differences in susceptibility results, which appear to be method related, can result when isolates of the B. fragilis group are tested. Therefore, studies correlating in vitro results, determined by various methods, to clinical outcome are essential.
Publisher
American Society for Microbiology
Reference13 articles.
1. Comparison of susceptibilities of anaerobic bacteria determined by agar dilution and by a microbroth method;Baron E. J.;Rev. Infect. Dis. l(Suppl.):S249-S253.,1984
2. Antibiotic sensitivity testing. Report of an international collaborative study;Ericsson H. M.;Acta Pathol. Microbiol. Scand. Supply.,1971
3. Finegold S. M. 1977. Anaerobic bacteria in human disease. Academic Press Inc. New York.
4. Holdeman L. V. E. P. Cato and W. E. C. Moore (ed.). 1977. Anaerobe laboratory manual 4th ed. Virginia Polytechnic Institute and State University Blacksburg.
5. Collaborative evaluation of the Micro-Media Systems anaerobe susceptibility panel: comparisons with reference methods and test reproducibiity;Jones R. N.;J. Clin. Microbiol.,1982
Cited by
40 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献