Affiliation:
1. Laboratory Sciences and Epidemiology Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dacca.
Abstract
A simple, rapid, and reliable method to detect Vibrio cholerae in fecal specimens would assist in the management of cases of severe diarrhea, especially since most such cases occur in areas with minimal laboratory facilities. A coagglutination test was used to detect V. cholerae antigen in bile-peptone broth incubated with feces. In the technique, Staphylococcus aureus Cowan 1 coated with anti-V. cholerae O1 antiserum was tested with cultures incubated for 4 h. When 165 specimens were tested, the sensitivity, specificity, and accuracy of the test, compared with standard culture methods, were 97, 99, and 98%, respectively. These promising results were better than those of dark-field microscopy using the same specimens, and the test was logistically easy to perform. The coagglutination test using enrichment broth culture of feces is a simple and rapid method which may be used to confirm a diagnosis of cholera.
Publisher
American Society for Microbiology
Reference15 articles.
1. Rapid identification of Vibrio cholerae by darkfield microscopy;Benenson A. S.;Bull. W. H. O.,1964
2. Detection of Salmonella typhi antigens by slide coagglutination in urine from patients with typhoid fever;Dhanalakshmi D.;Indian J. Pathol. Microbiol.,1984
3. Method for identifying Salmonella and Shigella directly from the primary isolation plate by coagglutination of protein A-containing staphylococci sensitized with specific antibody;Edwards E. A.;J. Clin. Microbiol.,1976
4. Rapid identification of cholera vibrios with fluorescent antibody;Finkelstein R. A.;J. Bacteriol.,1959
5. Fung J. C. and R. C. Tilton. 1985. Detection of bacterial antigens by counterimmunoelectrophoresis coagglutination and latex agglutination p. 883-890. In E. H. Lennette A. Balows W. J. Hausler Jr. and H. J. Shadomy (ed.) Manual of clinical microbiology 4th ed. American Society for Microbiology Washington D.C.
Cited by
49 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献