PCR Amplification and Phylogenetic Analysis of groESL Operon Sequences from Ehrlichia ewingii and Ehrlichia muris

Author:

Sumner John W.1,Storch Gregory A.2,Buller Richard S.2,Liddell Allison M.2,Stockham Steven L.3,Rikihisa Yasuko4,Messenger Sharon1,Paddock Christopher D.1

Affiliation:

1. Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia1;

2. The Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine and St. Louis Children's Hospital, St. Louis, Missouri2;

3. Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, Missouri3; and

4. Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio4

Abstract

ABSTRACT Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii , recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris . Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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