False-Positive Results for Mycobacterium celatum with the AccuProbe Mycobacterium tuberculosis Complex Assay

Author:

Somoskövi Ákos12,Hotaling Jacqueline E.1,Fitzgerald Marie1,Jonas Vivian3,Stasik Denise1,Parsons Linda M.1,Salfinger Max14

Affiliation:

1. Wadsworth Center, New York State Department of Health,1 and

2. Department of Respiratory Medicine, Semmelweis University, Budapest, Hungary2; and

3. Gen-Probe Incorporated, San Diego, California3

4. Department of Medicine, Albany Medical College,4 Albany, New York;

Abstract

ABSTRACT Mycobacterium celatum type 1 was found to cross-react in the AccuProbe Mycobacterium tuberculosis complex assay. Subsequently, we found a statistically significant increase in the relative light units with lower temperatures, suggesting that it is necessary to perform this AccuProbe assay at between 60 and 61°C. We also recommend the inclusion of M. celatum type 1 as a negative control.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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