A Method for Variant Agnostic Detection of SARS-CoV-2, Rapid Monitoring of Circulating Variants, and Early Detection of Emergent Variants Such as Omicron-Reference-Cited by-同舟云学术

A Method for Variant Agnostic Detection of SARS-CoV-2, Rapid Monitoring of Circulating Variants, and Early Detection of Emergent Variants Such as Omicron

Published:2022-07-20 Issue:7 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lai Eric¹,Kennedy Emily B.²,Lozach Jean³,Hayashibara Kathleen⁴,Davis-Turak Jeremy³,Becker David⁵,Brzoska Pius⁴,Cassens Tyler⁵,Diamond Evan⁴,Gandhi Manoj⁴,Greninger Alexander L.⁶⁷^ORCID,Hajian Pooneh⁶,Leonetti Nicole A.⁵,Nguyen Jason M.⁵,O’Donovan K. M. Clair⁸,Peck Troy⁵,Ramirez Jimmy M.⁵,Roychoudhury Pavitra⁶⁷^ORCID,Sandoval Efren⁵,Wesselman Cassandra³,Wesselman Timothy³,White Simon⁵,Williams Stephen⁴,Wong David⁵,Yu Yufei⁸,Creager Richard S.⁹^ORCID

Affiliation:

1. Personalized Science, Burlington, Vermont, USA

2. OOMVELT, Lakewood, Ohio, USA

3. ROSALIND, San Diego, California, USA

4. Thermo Fisher Scientific, South San Francisco, California, USA

5. Helix OpCo, San Diego, California, USA

6. University of Washington Medical Center, Seattle, Washington, USA

7. Fred Hutchinson Cancer Research Center, Seattle, Washington, USA

8. Biocomx, Dana Point, California, USA

9. Seegene Technologies, Walnut Creek, California, USA

Abstract

The rapid emergence of SARS-CoV-2 variants raised public health questions concerning the capability of diagnostic tests to detect new strains, the efficacy of vaccines, and how to map the geographical distribution of variants to understand transmission patterns and loads on healthcare resources. Next-generation sequencing (NGS) is the primary method for detecting and tracing new variants, but it is expensive, and it can take weeks before sequence data are available in public repositories.

Funder

HHS | NIH | National Institute of Biomedical Imaging and Bioengineering

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.00342-22

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