Comparison between two methods of assaying relative microbial activity in marine environments

Author:

Griffiths R P,Hayasaka S S,McNamara T M,Morita R Y

Abstract

Two methods for determining relative microbial activity in the marine environment were compared. In one method, a single concentration of a labeled substrate was used to calculate rates of substrate utilization; in the other, multiple concentrations of the same substrate (heterotrophic activity method) were used to calculate maximum potential substrate utilization rates. These studies were made on 232 seawater and 79 sediment samples taken from a variety of marine environments. The highest correlations between these two methods were seen in the sediment samples tested. The lowest correlation coerfficient seen in the sediment samples was 0.90, and the highest was 0.98. In seawater samples (six studies), the lowest correlation coefficient was 0.77 and the highest was 0.95. The correlation between these two methods was also substrate concentration dependent. Higher correlation coefficients were observed when higher substrate concentrations were used. Under certain conditions, these two methods appear to be comparable for estimating relative levels of microbial activity in the marine environment.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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5. Wright R. T. 1973. Some difficulties in using "4C organic solutes to measure heterotrophic bacterial activity 199-217. In H. R. Stevenson and R. R. Colwell (ed.) Proceeding of the Belle W. Baruch Symposium on Marine Science and Estuarine Microbial Ecology. University of South Carolina Press Columbia.

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