Affiliation:
1. Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan
Abstract
ABSTRACT
We report here the first analysis of
Erysipelothrix
spp. using pulsed-field gel electrophoresis (PFGE). Seventy strains of
Erysipelothrix
spp. were analyzed.
Sma
I,
Asc
I, and
Not
I were tested for the ability to cleave the DNA extracted from those strains, and among them,
Sma
I was the most reliable enzyme. Sixty-three distinct PFGE patterns were produced, and no DNA degradation was observed, allowing the identification of all of the strains. Based on these results and on those of a previous analysis using randomly amplified polymorphic DNA and ribotyping, PFGE with
Sma
I might be considered to be more sensitive than those methods and to be the best method for epidemiological studies of strains of this genus.
Publisher
American Society for Microbiology
Cited by
23 articles.
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