Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism of gap Gene-Reference-Cited by-同舟云学术

Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism of gap Gene

Published:2001-10 Issue:10 Volume:39 Page:3693-3695
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Yugueros Javier¹,Temprano Alejandro¹,Sánchez Marı́a¹,Luengo José Marı́a²,Naharro Germán¹

Affiliation:

1. Departamento de Sanidad Animal, Microbiologı́a e Inmunologı́a1 and

2. Departamento de Bioquı́mica y Biologı́a Molecular,2 Universidad de León, 24071 León, Spain

Abstract

ABSTRACT Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, Alu I digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.10.3693-3695.2001

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