Affiliation:
1. Department of Microbiology, Wayne State University College of Medicine, Detroit, Michigan
Abstract
Eaves, George
N. (Wayne State University College of Medicine, Detroit, Mich.)
and Charles D. Jeffries
. Isolation and properties of an exocellular nuclease of
Serratia marcescens
. J. Bacteriol.
85:
273–278. 1963.—The exocellular nuclease of
Serratia marcescens
, isolated by anion-exchange chromatography on diethylaminoethyl-Sephadex, depolymerized deoxyribonucleic acid, ribonucleic acid, and the polynucleotide which is refractory to pancreatic ribonuclease activity. The enzyme was tentatively classified as a nonspecific phosphodiesterase. Magnesium was essential for activity, which was optimal at pH 8.8. The purified enzyme was completely inactivated by heating at 50 C for 15 min.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
94 articles.
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