Arbitrary primer polymerase chain reaction, a powerful method to identify Bacillus thuringiensis serovars and strains

Author:

Brousseau R1,Saint-Onge A1,Préfontaine G1,Masson L1,Cabana J1

Affiliation:

1. Biotechnology Research Institute, National Research Council of Canada, Montreal.

Abstract

Arbitrary primer polymerase chain reaction technology has been applied to the identification of commercial strains of Bacillus thuringiensis by using total DNAs extracted from single bacterial colonies as templates. Characteristic DNA banding patterns can be readily and reproducibly obtained by agarose gel electrophoresis. This method has been used to distinguish commercial products containing B. thuringiensis serovar kurstaki (3a3b). When a single primer was used this method was capable of producing discriminating DNA fingerprints for 33 known serovars. Differentiation from the closely related species Bacillus cereus is also readily achieved. This technique should prove to be a powerful tool for identification and discrimination of individual B. thuringiensis strains.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference26 articles.

1. Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16S rRNA;Ash C.;Int. J. Syst. Bacteriol.,1991

2. Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis;Battisti L.;J. Bacteriol.,1985

3. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers;Caetano-Anolles G.;Bio/Technology,1991

4. de Barjac H. 1981. Identification of H-serotypes of Bacillus thuringiensis p. 35-43. In H. D. Burges (ed.) Microbial control of pest and plant diseases 1970-1980. Academic Press Inc. (London) Ltd. London.

5. Doyle R. J. K. F. Keller and J. W. Ezzell. 1985. Bacillus p. 211-215. In E. H. Lennette (ed.) Manual of clinical microbiology 4th ed. American Society for Microbiology Washington D.C.

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