Effects of Preservatives on Viability of Cryptosporidium parvum Oocysts-Reference-Cited by-同舟云学术

Effects of Preservatives on Viability of Cryptosporidium parvum Oocysts

Published:1993-12 Issue:12 Volume:59 Page:4361-4362
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Campbell A. T.¹,Robertson L. J.¹,Smith H. V.¹

Affiliation:

1. Scottish Parasite Diagnostic Laboratory, Stobhill General Hospital, Springburn, Glasgow G21 3UW, and Division of Environmental Health, University of Strathclyde, Glasgow G1 1XQ, 2 Scotland

Abstract

Potassium dichromate and formalin reduced the viability of Cryptosporidium parvum oocysts as assessed by inclusion or exclusion of 4′,6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) and excystation. Some formalin-treated oocysts containing dead sporozoites excluded PI; that this fluorogenic assay relies not solely upon exclusion of PI but also upon highlighting of sporozoite nuclei by DAPI is reiterated.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.59.12.4361-4362.1993

Reference12 articles.

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2. Blewett D. A. 1989. Disinfection and oocysts p. 107-116. In K. W. Angus and D. A. Blewett (ed.) Cryptosporidiosis. Proceedings of the First International Workshop. The Animal Disease Research Association Edinburgh.

3. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes;Campbell A. T.;Appl. Environ. Microbiol.,1992

4. Effects of disinfectants on survival of cryptosporidium oocysts;Campbell I.;Vet. Rec.,1982

5. Cryptosporidium: its biology and potential for environmental transmission;Current W. L.;Crit. Rev. Environ. Control,1986

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