Affiliation:
1. Department of Developmental Immunology, Max Planck Institute for Immunobiology, D-79108 Freiburg, Germany
Abstract
ABSTRACT
The
Capn5
gene was inactivated by homologous recombination in ES cells that subsequently colonized the germ line of mice. The targeted mutation integrated a
lacZ
expression cassette into the
Capn5
gene, allowing the expression of
Capn5
mRNA to be examined in detail in heterozygous animals. Expression was observed in embryonic and newborn thymuses, in various epithelial tissues, and in tissues of the central nervous system. In the thymus,
Capn5
was expressed mainly in relatively immature CD25
+
embryonic thymocytes. Despite the numerous expression sites of
Capn5
, the majority of
Capn5
-null mice were viable and fertile and appeared healthy. Histopathological analysis did not reveal any differences between
Capn5
-null and wild-type mice. There were no defects in the major T- or B-cell populations in the thymus, spleen, bone marrow, or peritoneum, nor did apoptosis appear abnormal in
Capn5
-null T cells. There was no evidence for the development of autoimmune disease in
Capn5
-null animals. However, a small proportion of homozygous null offspring from heterozygous matings were runted and most often did not survive to adulthood.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
21 articles.
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