Affiliation:
1. Departamento de Patologı́a Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Abstract
ABSTRACT
A PCR-based method was developed for the specific detection of
Yersinia ruckeri
in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (
n
= 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of
Y. ruckeri
in infected trout tissues.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference22 articles.
1. Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water
2. Austin
B.
Austin
D. A.
Bacterial fish pathogens: diseases in farmed and wild fish
2nd ed.
1993
208
216
Ellis Horwood Limited
Chichester United Kingdom
3. Establishment of an asymptomatic carrier state infection of enteric redmouth disease in rainbow trout (Salmo gairdneri).;Busch R. A.;J. Fish Res. Board Can.,1975
4. Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification
5. Molecular identification of pathogenic actinomycetes: a new approach.;Fernández-Garayzábal J. F.;Microbiol. SEM,1995
Cited by
64 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献