Engineering of a Stable Whole-Cell Biocatalyst Capable of ( S )-Styrene Oxide Formation for Continuous Two-Liquid-Phase Applications-Reference-Cited by-同舟云学术

Engineering of a Stable Whole-Cell Biocatalyst Capable of ( S )-Styrene Oxide Formation for Continuous Two-Liquid-Phase Applications

Published:1999-12 Issue:12 Volume:65 Page:5619-5623
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Panke Sven¹,de Lorenzo Víctor²,Kaiser Arnë¹,Witholt Bernard¹,Wubbolts Marcel G.¹

Affiliation:

1. Institute of Biotechnology, Swiss Federal Institute of Technology Zurich, 8093 Zurich, Switzerland,1 and

2. Centro Nacional de Biotecnologia, Campus de Cantoblanco, 28049 Madrid, Spain2

Abstract

ABSTRACT Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia coli recombinants. Such strains maintained the introduced styrene oxidation activity in continuous two-liquid-phase cultures for at least 100 generations, although at a lower level than in the shaking-flask experiments. The data suggest that placement of target genes on the chromosome might be a suitable route for the construction of segregationally stable and highly active whole-cell biocatalysts.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.12.5619-5623.1999

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