Eucaryotic Diversity in a Hypersaline Microbial Mat

Author:

Feazel Leah M.1,Spear John R.2,Berger Alicia B.3,Harris J. Kirk1,Frank Daniel N.1,Ley Ruth E.4,Pace Norman R.1

Affiliation:

1. Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347

2. Division of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401

3. Cancer Biology Program and Pathology Department, School of Medicine, Stanford University, Palo Alto, California 94305

4. School of Medicine, Washington University, St. Louis, Missouri 63108

Abstract

ABSTRACT To determine the eucaryotic diversity of the hypersaline Guerrero Negro microbial mat, we amplified 18S rRNA genes from DNA extracted from this mat and constructed and analyzed clone libraries. The extent of eucaryotic diversity detected was remarkably low, only 15 species among 890 clones analyzed. Six eucaryotic kingdoms were represented, as well as a novel cluster of sequences. Nematode sequences dominated the clone libraries.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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