Establishment of a Gene Expression System in Ochrobactrum anthropi-Reference-Cited by-同舟云学术

Establishment of a Gene Expression System in Ochrobactrum anthropi

Published:2006-10 Issue:10 Volume:72 Page:6833-6836
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Seleem Mohamed N.¹,Ali Mohammed²,Boyle Stephen M.¹,Mukhopadhyay Biswarup³,Witonsky Sharon G.¹,Schurig Gerhardt G.¹,Sriranganathan Nammalwar¹

Affiliation:

1. Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine

2. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

3. Virginia Bioinformatics Institute, Virginia Polytechnic and State University, Blacksburg, Virginia

Abstract

ABSTRACT Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01446-06

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